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KMID : 0525619980030010006
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1998 Volume.3 No. 1 p.6 ~ p.18
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Development of Microbial Chitosanases for the Production of High DP Chitooligosaccharides
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정미라/Jung MR
조유영/지연태/박노동/Jo YY/Chi YT/Park RD
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Abstract
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In order to obtain microbial endochitosanases for enzymatic production of high dp chitooligosaccharides from chitosan we screended 9 microbes which showed high chitinolytic activities. We tested the chitosanase activities of total 32 enzymes including 23 commercial enzymes and 9 crude enzymes produced by the microbes, in terms of the decreases in turbidity and viscosity of chitosan solution, the amount of pecipitation after neutralization, and reducing sugar-producing activity, after incubation with each enzyme. We selected Bacillus spp. P20, P21, and P30 as potential sources for production of endochitosanases to produce high dp chitooligosaccharides. Among them, enzyme produced by P2l was most potent and comparable to commercial enzyme E7. The culture supernatant of the strain P2l showed strong liquefaction activity and rapid decrease in viscosity of chitosan solution. By TLC and HPLC, chitooligosaccharides of dp 2-7 were separated and identified from the enzyme hydrolyzates of chitosn. The chitosanase from Bacillus sp. P2l was thus regarded as an endo-splitting and applicable to enzymatic production of high dp chitooligasaccharides from chitosan.
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